Plant Transcription Factor Database
Previous version: v3.0
Capsella rubella
HSF Family
Species TF ID Description
Carubv10001065mHSF family protein
Carubv10001204mHSF family protein
Carubv10003259mHSF family protein
Carubv10004729mHSF family protein
Carubv10004840mHSF family protein
Carubv10004953mHSF family protein
Carubv10005408mHSF family protein
Carubv10006025mHSF family protein
Carubv10006026mHSF family protein
Carubv10007111mHSF family protein
Carubv10007333mHSF family protein
Carubv10007409mHSF family protein
Carubv10007768mHSF family protein
Carubv10008952mHSF family protein
Carubv10009522mHSF family protein
Carubv10013628mHSF family protein
Carubv10013808mHSF family protein
Carubv10014189mHSF family protein
Carubv10014950mHSF family protein
Carubv10019491mHSF family protein
Carubv10020559mHSF family protein
Carubv10021131mHSF family protein
Carubv10023839mHSF family protein
Carubv10024599mHSF family protein
Carubv10025710mHSF family protein
Carubv10026722mHSF family protein
Carubv10026734mHSF family protein
Carubv10026842mHSF family protein
Carubv10027643mHSF family protein
Carubv10028300mHSF family protein
Carubv10028638mHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645